上睑下垂
炎症体
内质网
肾
细胞凋亡
医学
切碎
未折叠蛋白反应
药理学
缺血
急性肾损伤
肾功能
肾缺血
内科学
再灌注损伤
内分泌学
炎症
化学
生物化学
作者
Jiarui Wang,Yingli Yu,Haorui Zhang,Lipeng Zhang,Jing Wang,Shijia Su,Yue Zhang,Lei Song,Kun Zhou
标识
DOI:10.1016/j.ejphar.2023.176187
摘要
Renal ischemia-reperfusion (I/R) is one of the main causes of acute kidney injury (AKI), for which there is currently no effective treatment. Recently, the interaction between endoplasmic reticulum (ER) stress and pyroptosis during AKI has been investigated. The purpose of this study was to investigate the protective effects of Gypenoside XVII (GP-17) against I/R-induced renal injury. In this study, mice were divided into 6 groups, sham group, I/R group, GP-17 low-, medium-, high-dose group, and positive control 4-PBA group. The renal I/R was performed in mice by clamping the bilateral renal pedicles for 40 min, and then reperfusing for 24 h. Blood and kidney samples were collected for analysis. The results showed that GP-17 improved renal function and alleviated renal histopathological abnormalities caused by I/R. In addition, GP-17 pretreatment significantly decreased the expression or phosphorylation of ER stress response proteins including BIP, p-PERK, and CHOP. Besides, GP-17 inhibited the expression of pyroptosis proteins including caspase-1, GSDMD, and apoptotic protein BAX. The inflammatory factor IL-1β in these GP-17 pretreatment groups was also significantly reduced. GP-17 blocked NLRP3 inflammasome activation by inhibiting ERS, thereby inhibiting renal tubular cell pyroptosis and apoptosis, and prevented renal I/R injury.
科研通智能强力驱动
Strongly Powered by AbleSci AI