巩膜
ATF6
未折叠蛋白反应
内质网
延伸率
体内
细胞生物学
眼科
化学
医学
生物
材料科学
遗传学
极限抗拉强度
冶金
作者
Shin‐ichi Ikeda,Michio Ohta,Xiaoyan Jiang,Yukihiro Miwa,Deokho Lee,Naho Serizawa,Heonuk Jeong,Kiwako Mori,Yusaku Katada,Hiromitsu Kunimi,Nobuhiro Ozawa,Chiho Shoda,Mari Ibuki,Kazuno Negishi,Hidemasa Torii,Michio Ohta
标识
DOI:10.1038/s41467-022-33605-1
摘要
Abstract Axial length is the primary determinant of eye size, and it is elongated in myopia. However, the underlying mechanism of the onset and progression of axial elongation remain unclear. Here, we show that endoplasmic reticulum (ER) stress in sclera is an essential regulator of axial elongation in myopia development through activation of both PERK and ATF6 axis followed by scleral collagen remodeling. Mice with lens-induced myopia (LIM) showed ER stress in sclera. Pharmacological interventions for ER stress could induce or inhibit myopia progression. LIM activated all IRE1, PERK and ATF6 axis, and pharmacological inhibition of both PERK and ATF6 suppressed myopia progression, which was confirmed by knocking down above two genes via CRISPR/Cas9 system. LIM dramatically changed the expression of scleral collagen genes responsible for ER stress. Furthermore, collagen fiber thinning and expression of dysregulated collagens in LIM were ameliorated by 4-PBA administration. We demonstrate that scleral ER stress and PERK/ATF6 pathway controls axial elongation during the myopia development in vivo model and 4-PBA eye drop is promising drug for myopia suppression/treatment.
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