Effect of Shengyu decoction on changing T cell subgroups and cytokine levels in cyclophamide-induced mice

Objective To investigatethe effectsofShengyudecoction (SYD)onTcellsubgroupsandcytokine levels in immuno deficiency mice. Methods Kunming type mice were injected with cyclophosphamide to establish immuno deficiency mice model. The immuno deficiency mice were fed with low dose, high dose SYD respectively,once a day. On the 30th day, the mice were weighted; blood and the plasma was separated to measure the cytokine levels by ELISA test, and the T cell subgroups of CD3+, CD4+and CD8+ were measured by flow cytometor analysis system. Results Cyclophosphamide remarkedly resulted in decreasing of CD3+T cell, CD4+T cell, CD4+/CD8+ratio, IL-2 and IL-4 production. SYD could increase the percentage of CD3+T cell, CD4+T cell and CD4+/CD8+ ratio obviously in immuno deficiency mice compared to those in cyclophosphamide group (p0.05). The plasma level of IL-2 and IL-4 of immune deficit mice could be significantly improved by SYD. Conclusion SYD can immunomodulate the unusual distribution caused by cyclophosphamide among T cell subgroups of the mice with low immunity, improve the percentage of CD3+T cell, CD4+T cell and the ratio of CD4+/CD8+in mice, and promote the secretion of IL-2, IL-4 cytokines in mice with low immunity.