Double-enzyme Method for Polysaccharides Extraction from Gomphidius rutilus Fruitbodies

For polysaccharide extraction,wild Gomphidius rutilus fruit bodies were hydrolyzed with cellulase and papain together.Based on single factor investigations,orthogonal array design was employed to optimize the hydrolysis process,and the optimum hydrolysis conditions were found as follows:hydrolysis temperature 60 ℃;cellulase/papain mass ratio 3:1;pH 5.0;and hydrolysis duration 3 h and the polysaccharide yield obtained under such conditions was 12.0%.This method has many advantages over conventional methods,such as gentle reaction conditions,time saving and less destructive effect on polysaccharide structure.