[Objective] The aim was to study the effects of blue light on cordycepin content and conidia amount of Cordyceps militaris mycelium.[Method] The cordycepin content of mycelium and amount of Cordyceps militaris conidia was detected at different blue light irradiation times by using Cordyceps militaris as materials.[Result] The production of cordycepin was inhibited to a certain degree and the changes of cordycepin content also had a certain fluctuation after the Cordyceps militaris was irradiated by blue light;the amount of conidia of Cordyceps militaris irradiated by blue light was more than its number in the darkness at the same sampling time,and the number of conidia had been on an upward trend during a definite period of time at the same time.[Conclusion] This study provided foundation for the system researches on the effects of blue light on Cordyceps militaris.