The effect of different types of enzymes,,hydrolysis time on antioxidant activity of whey protein hydrolysate were studied in this paper.The results showed that the optimum two enzyme hydrolysis conditions respectively was: pepsin 0.15 %,37 ℃,hydrolysis time 2 h;trypsin 0.4 %,50 ℃,hydrolysis time 2 h,while the whey protein hydrolysate has a good antioxidant activity.