Tyrosine phosphorylation of proteins in murine thymocytes-a comparison between immunoprecipitation and Western blotting

A comparison between the two methods was performed for tyrosine phosphorylation of proteins in murine thymocytes exposed to low dose radiation using antiphosphotyrosine McAb.It was shown that tyrosine phosphorylation of 17.5 kD,22 kD,28 kD,32 kD,40 kD,43 kD and 53 kD protein molecules labelled metabolically with H332PO4 appeared in the thymocytes after radiation by immunoprecipitation technique and tyrosine phosphorylation of 28 kD,32 kD protein molecules appeared by western blotting.The results suggest that immunoprecipitation is superior to Western blotting in detection of protein tyrosine phosphorylation.