TWO PRESERVATION METHODS OF TOXOPLASMA GONDII

Objective To seek a simple proper preservation method of Toxoplasma gondii strains and provide foundation for further study.Methods Cryopreservation of Toxoplasma gondii strains by using refrigerator in different temperature and cell culture conservation were tested.Results Toxoplasma gondii can be preserved at(-196°C,)(-70°C) permanently,and(4°C) for 14 days.Plenty of Toxoplasma gondii can be obtained by cell culture and it can infect almost every kinds of cells except erythroplastid.Conclusion The virulence of Toxoplasma gondii was steady after two preservation methods.