圆二色性
适体
化学
单体
焓
热稳定性
赫帕
G-四倍体
DNA
结晶学
色谱法
聚合物
生物化学
分子生物学
有机化学
生物
滤波器(信号处理)
物理
量子力学
计算机科学
计算机视觉
作者
Slavomíra Poniková,Marián Antalík,Tibor Hianik
出处
期刊:General Physiology and Biophysics
[AEPress, s.r.o.]
日期:2008-12-01
卷期号:27 (4): 271-7
被引量:7
摘要
The effect of K+ and Na+ on the properties of DNA aptamers that selective binds to fibrinogen (FIBRI) and heparin (HEPA) exosites of human alpha-thrombin was studied by circular dichroism (CD). The complexes of FIBRI-K+ were slightly more stable than HEPA-K+. However, lower stability was observed for HEPA-K+ at presence of Na+ in comparison with FIBRI-K+. The analysis of CD melting curves suggests differences in thermal stability of both aptamers at presence of K+. The melting temperatures (Tm) and changes in van't Hoff enthalpy for HEPA-K+ complexes were lower in comparison with those for FIBRI-K+. With increasing HEPA concentration the Tm value increased, but Tm did not change with increasing FIBRI concentration. This suggests formation of HEPA aggregates, while FIBRI aptamers are in monomeric form.
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