色谱法
胶束电动色谱
化学
堆积
电动现象
体积热力学
分析化学(期刊)
检出限
量子力学
物理
物理化学
有机化学
作者
Yu‐Xiu Shao,Guanhua Chen,Rou Fang,Li Zhang,Ling‐Xiao Yi,Hong-Lian Meng
标识
DOI:10.1021/acs.jafc.6b00482
摘要
A new micellar electrokinetic chromatography method with large-volume sample stacking and polarity switching was developed to analyze amoxicllin, cephalexin, oxacillin, penicillin G, cefazolin, and cefoperazone in milk and egg. The important parameters influencing separation and enrichment factors were optimized. The optimized running buffer consisted of 10 mM phosphate and 22 mM SDS at pH 6.7. The sample size was 1.47 kPa × 690 s, the reverse voltage was 20 kV, and the electric current recovery was 95%. Under these optimum conditions, the enrichment factors of six β-lactams were 193-601. Their LODs were <0.26 ng/g, and LOQs were all 2 ng/g, which was only 1/50-1/2 of the maximum residual limits demanded by U.S. and Japanese regulations. The intraday and interday RSDs of method were lower than 3.70 and 3.91%, respectively. The method can be applied to determine these six antibiotic residues in egg and milk.
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