Protective effects of total flavones from Lophatherum gracile on myocardial ischemia-reperfusion injury in rats

Aim To study the protective effect of total flavones from Lophatherum gracile(TFLG) on myocardial ischemia-reperfusion injury in rats.Methods The ischemic model was made by occluding the anterior descending of the left artery(LAD) in rats.The activity of lactate dehydrogenase(LDH),creatine kinase(CK),levels of the superoxide dismutase(SOD),maleic dialdehyde(MDA),glutathione peroxidase(GSH-PX) and nitric oxide(NO) in serum and myocardial tissue were measured.Results TFLG(50 and 100 mg·kg-1) obviously reduced the activity of CK and LDH,decreased the level of MDA and increased the content of SOD,GSH-PX and NO.TFLG(100 mg·kg-1) inhibited the expression of NF-κB and TNF-α and reduced the expression of caspase-3 protein.Conclusion TFLG has certain protective effect on myocardial ischemia-reperfusion injury in rats via attenuating oxygen free radical,inhibiting inflammatory reaction and reducing apoptosis.