A Contrast of Chlorogenic Acid and Rutin in Different Parts of Galium verum L.

[Objective] To compare the content of chlorogenic acid and rutin in the different parts of Galium verum L.by using the HPLC-DAD method.[Method] A HPLC-DAD method was developed for the content determination of rutin and chlorogenic acid in root,stem,leafage and flower from Galium verum L..[Result] A good linearity was obtained,the linear range were: rutin 0.024 3-1.950 0 μg,chlorogenic acid 0.132 5-10.600 0 μg;the average recovery of rutin was 101.02% and the average recovery of chlorogenic acid was 101.65%.The content of rutin and chlorogenic acid was the highest in flowers and the lowest in roots.[Conclusion] The HPLC-DAD method separation is good,stable and can be used for the quality control of Galium verum L.,the contents of rutin and chlorogenic acid are different in the different parts of Galium verum L..