Comparison of the automatic immunohistochemistry stainer and the manual immunostaining

Objective To observe and compare the application between Ventana automatic immunohistochemistry stainer and the manual immunostaining.Methods Several kinds of primary antibodies which were applied to the routine work and the same secondary antibodies were stained by the machine and manual immunohistochemistry operation respectively,and were compared.Results The active results stained by automatic immunohistochemistry stainer of which every index was prior to the manual operation were shown to the well-distributed coloration,exact location,non-edge-effect and clear background.Conclusion The automatic immunohistochemistry stainer is not only high-degree standardizaion and good to normative operation,but also reduces the personal error in manual staining.It could provide exact evidence for clinical pathological diagnosis.