Rapid Determination of Vitamin B_6 with the MHEBM as a Fluorescent Molecule Probe

A new method for determination of vitamin B_6(VB_6) is developed based on the fluorescence enhancement of 6-methyl-2-(α-hydroxyethyl)-1H-benzimidazole(MHEBM) by VB_6 with the MHMBM as a novel fluorescent molecule probe.The experimental results show that the linear relationship between the fluorescence enhancement effect of MHMBM and the concentration of VB_6 is good in neutral aqueous solutions.The correlation coefficient (r) and the linear range are 0.999 8 and(0.2—18.2)μg/mL respectively with the detection limit of 0.098μg/ mL.The method has been applied to determination of VB_6 in sample with 2.32%RSD.The recovery is 99.54% and the results are satisfactory.