Effects of temperature,time and solid-liquid ratio on the extraction of β-carotene from Rhodotorula benthica(HN-3) were studied by DMSO method to find out the optimum extraction technology and provide a reliable theoretical basis for industrial production of β-carotene from Rhodotorula benthica.The results showed that the optimum conditions included solid-liquid ratio 1∶25,extraction time 60 min and extraction temperature 30℃,the amount of β-carotene could be up to 345.7μg/g,10.93% higher than that extracted under single factor condition(307.9μg/g).The optmium extraction technology laid the foundation for efficient extraction of β-carotene from Rhodotorula benthica.