骨髓生成
生物
骨髓
中国仓鼠卵巢细胞
红细胞生成
免疫学
粒细胞
乳铁蛋白
同源染色体
内分泌学
分子生物学
内科学
造血
细胞生物学
细胞培养
生物化学
贫血
干细胞
医学
基因
遗传学
作者
Michał Zimecki,Jolanta Artym,Maja Kocięba,Katarzyna Kaleta-Kuratewicz,Piotr Kuropka,Jan Kuryszko,Marian L. Kruzel
出处
期刊:Stem Cells and Development
[Mary Ann Liebert]
日期:2013-12-15
卷期号:22 (24): 3261-3270
被引量:7
标识
DOI:10.1089/scd.2013.0242
摘要
The effects of lactoferrin (LF), an iron binding protein, on myelopoiesis have been studied extensively in vitro and in vivo in human and murine models over the past three decades. Due to the lack of high-quality homologous LFs, however, the conclusions are still unequivocal. Recently, both human and murine LFs have become available as recombinant products expressed in Chinese hamster ovary (CHO) cell lines showing mammalian type of glycosylation, thus apparently species compatible. In this study, we present the effects of homologous recombinant mouse LF (rmLF) on myelopoiesis in CBA mice. The myelocytic lineage has been assessed by their appearance in circulating blood and bone marrow, and induction of relevant mediators of inflammation. Intravenous injection of rmLF (100 μg/mouse) resulted in a significantly increased number of myelocytic cells in the circulating blood after 24 h. Mouse serum transferrin, used as a control protein, showed no stimulatory effect. The increase in output of neutrophil precursors, neutrophils, and eosinophils was correlated with a twofold increase of leukocyte concentrations. The analysis of the bone marrow sections confirmed increased myelopoiesis. The alterations in the bone marrow cell composition were statistically significant regarding mature neutrophils (10.8% vs. 27.7%), metamyelocytes (11.4% vs. 16.0%), and myelocytes (2.4% vs. 4.0%). The mobilization of the myelocytic cells in the bone marrow and the increased output of these cells into circulation were accompanied by elevated serum concentrations of interleukin-6 at 6 h and haptoglobin at 24 h following administration of rmLF. In conclusion, the homologous LF elicits significant and transient myelopoiesis in experimental mice.
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