Inhibiting miRNA Function by Antisense Oligonucleotides in Cultured Mammalian Cells

Methods for transfecting antisense oligonucleotides (ASOs) into mammalian cells for suppression of miRNA function are described in this protocol. The effects of ASOs on the miRNA are evaluated by measuring the protein level of the putative miRNA target or the activity of a reporter bearing a 3' untranslated region (3' UTR) derived from the putative miRNA target.