牙本质涎磷蛋白
细胞生物学
间充质干细胞
化学
鱼腥草素骨
干细胞
牙本质形成
牙乳头
碱性磷酸酶
成牙本质细胞
生物
牙本质
病理
骨钙素
医学
生物化学
酶
作者
Haoqing Yang,Yuncun Liang,Yangyang Cao,Yu Cao
摘要
Abstract Enhancing the functions of mesenchymal stem cells (MSCs) is considered a potential approach for promoting tissue regeneration. In the present study, we investigate the role of HOXC8 in regulating differentiation and migration by using stem cells of the apical papilla (SCAPs). Our results showed that overexpression of HOXC8 suppressed the osteo‐/dentinogenic differentiation, as detected by measuring alkaline phosphatase activity, in vitro mineralization, and the expressions of dentin sialophosphoprotein, dentin matrix acidic phosphoprotein 1, bone sialoprotein, runt‐related transcription factor 2, and osterix in SCAPs, and inhibited in vivo osteo‐/dentinogenesis of SCAPs. In addition, knockdown of HOXC8 promoted the osteo‐/dentinogenic differentiation potentials of SCAPs. Mechanically, HOXC8 enhanced KDM1A transcription by directly binding to its promoter. HOXC8 and KDM1A also inhibited the migration and chemotaxis abilities of SCAPs. To sum up, HOXC8 negatively regulated the osteo‐/dentinogenic differentiation and migration abilities of SCAPs by directly enhancing KDM1A transcription and indicated that HOXC8 and KDM1A could serve as potential targets for enhancing dental MSC mediated tissue regeneration.
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