LBA01-01 FUNGI AND BACTERIA ENTOMBED WITHIN HUMAN CALCIUM OXALATE-APATITE KIDNEY STONES

You have accessJournal of UrologyLate-Breaking Abstracts (LBA1)1 Apr 2020LBA01-01 FUNGI AND BACTERIA ENTOMBED WITHIN HUMAN CALCIUM OXALATE-APATITE KIDNEY STONES Jessica Saw*, Mayandi Sivaguru, Elena Wilson, Yiran Dong, Rob Sanford, Christopher Fields, Melissa Cregger, William Bruce, Annette Merkel, John Lieske, Amy Krambeck, Marcelino Rivera, Tim Large, Nicholas Chia, Michael Romero, Dirk Lange, Joe Weber, and Bruce Fouke Jessica Saw*Jessica Saw* More articles by this author , Mayandi SivaguruMayandi Sivaguru More articles by this author , Elena WilsonElena Wilson More articles by this author , Yiran DongYiran Dong More articles by this author , Rob SanfordRob Sanford More articles by this author , Christopher FieldsChristopher Fields More articles by this author , Melissa CreggerMelissa Cregger More articles by this author , William BruceWilliam Bruce More articles by this author , Annette MerkelAnnette Merkel More articles by this author , John LieskeJohn Lieske More articles by this author , Amy KrambeckAmy Krambeck More articles by this author , Marcelino RiveraMarcelino Rivera More articles by this author , Tim LargeTim Large More articles by this author , Nicholas ChiaNicholas Chia More articles by this author , Michael RomeroMichael Romero More articles by this author , Dirk LangeDirk Lange More articles by this author , Joe WeberJoe Weber More articles by this author , and Bruce FoukeBruce Fouke More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000000946.01AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Introduction: Promotion and/or inhibition of key events during kidney stone growth over time may be linked to the physical presence of microorganisms, their metabolic functions and the proteins they produce. Our team has employed a cross-disciplinary integration of conceptual approaches and techniques in geology, biology and medicine (called GeoBioMed) to elucidate these processes. Methods: Kidney stones composed of both calcium oxalate and apatite (n=18) were removed from patients undergoing percutaneous nephrolithotomy. Bacterial and fungal 16S rRNA gene sequences and internal transcribed spacer (ITS) regions were amplified from whole stone genomic DNA on a FluidigmTM system and then paired-end sequenced on an IlluminaTM MiSeq. The datasets were denoised and analyzed with DADA2 software. Phylogenetic diversity estimates and statistical analysis were completed using Phyloseq v1.22.3 and R. Doubly polished petrographic 25 µm-thick thin sections were analyzed using brightfield, confocal and super-resolution autofluorescence microscopy to identify crystalline architecture and entombed organic matter. Results: A low-diversity community of entombed microbes were detected in all 18 stones, including bacterial sequences affiliated with the phyla Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria and fungal sequences affiliated with Aspergillus niger. Fungal sequences were entombed in 9 of 18 stones and correlated with higher urine calcium concentrations (335 ± 131 mg, p=0.01). Thin section petrography identified one calcium oxalate-apatite stone (of a total of 30 stones analyzed) that contained distinct and well-preserved fungal borings within apatite spherules (Fig. 1). Conclusions: A role for Aspergillus niger in human kidney stone mineral precipitation, secondary erosion and biogeochemical recycling is consistent with documented fungal activity in all natural environmental systems. These unexpected observations suggest a possible role for an entombed microbiome in human stone pathogenesis. Source of Funding: Mayo Clinic and University of Illinois Strategic Alliance for Technology-Based Healthcare; Mayo Clinic O’Brien Urology Research Center (No. DK100227); Mayo Clinic Center for Individualized Medicine; National Aeronautics and Space Administration (NASA) Astrobiology Institute (Cooperative Agreement No. NNA13AA91A) © 2020 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 203Issue Supplement 4April 2020Page: e1019-e1019 Advertisement Copyright & Permissions© 2020 by American Urological Association Education and Research, Inc.MetricsAuthor Information Jessica Saw* More articles by this author Mayandi Sivaguru More articles by this author Elena Wilson More articles by this author Yiran Dong More articles by this author Rob Sanford More articles by this author Christopher Fields More articles by this author Melissa Cregger More articles by this author William Bruce More articles by this author Annette Merkel More articles by this author John Lieske More articles by this author Amy Krambeck More articles by this author Marcelino Rivera More articles by this author Tim Large More articles by this author Nicholas Chia More articles by this author Michael Romero More articles by this author Dirk Lange More articles by this author Joe Weber More articles by this author Bruce Fouke More articles by this author Expand All Advertisement PDF downloadLoading ...