溶瘤病毒
上睑下垂
水泡性口炎病毒
生物
细胞毒性T细胞
免疫检查点
免疫系统
癌症研究
细胞凋亡
病毒学
免疫学
病毒
程序性细胞死亡
免疫疗法
体外
生物化学
作者
Jing Lin,Fei Liu,Fengmei Gao,Yujia Chen,Renling Wang,Xinyue Wang,Yue Li,Qi Li,Shihui Sun,Zi Li,Yungang Lan,Huijun Lu,Wei Guo,Li Du,Feng Gao,Deguang Song,Kui Zhao,Jiyu Guan,Wenqi He
标识
DOI:10.1016/j.bbadis.2022.166538
摘要
Traditionally, vesicular stomatitis virus (VSV) and other oncolytic viruses (OVs) are thought to kill tumors by inducing apoptosis. However, cell apoptosis leads to immune quiescence, which is incompatible with the ability of OVs to activate the antitumor immune microenvironment. Thus, studying OVs-mediated oncolytic mechanisms is of great importance for the clinical application of OVs.We examined the pyroptosis in tumor cells and tissues by morphological observation, Lactate Dehydrogenase (LDH) assay, frozen section observation, and western-blotting techniques. The critical role of GSDME in VSV-induced pyroptosis was confirmed by CRISPR/Cas9 technique. VSV virotherapy-recruited cytotoxic lymphocytes in the tumors were examined by flow cytometry assay. VSV-activated antitumor immunity was further enhanced by the co-administration with anti-PD-1 antibody.Here, we observed that VSV was able to trigger tumor pyroptosis through Gasdermin E (GSDME) in tumor cells, human tumor samples, and tumor-bearing mouse models. Importantly, the effectiveness of VSV-based virotherapy is highly dependent on GSDME, as depletion of GSDME not only reverses VSV-induced tumor-suppressive effects but also diminishes the ability of VSV to activate antitumor immunity. Notably, VSV treatment makes immunologically 'cold' tumors more sensitive to checkpoint blockade.Oncolytic VSV induces tumor cell pyroptosis by activating GSDME. GSDME is critical in recruiting cytotoxic T lymphocytes in the context of VSV therapy, which can switch immunologically 'cold' tumors into 'hot' and enhance immune checkpoint therapy efficacy.
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