辣根过氧化物酶
过氧化氢
过氧化物酶
中心组合设计
复合数
胶体金
化学
纳米颗粒
纳米技术
材料科学
色谱法
生物化学
酶
响应面法
复合材料
作者
Natchanok Talapphet,Chang Soon Huh
出处
期刊:Nano express
[IOP Publishing]
日期:2024-02-12
卷期号:5 (1): 015012-015012
标识
DOI:10.1088/2632-959x/ad246c
摘要
Abstract The oxidizing agent, hydrogen peroxide (H 2 O 2 ), which is a part of reactive oxygen species (ROS) is well-known to contribute to oxidative stress-induced damage to biological molecules. An excess of free radicals can harm health and is associated with human diseases. Gold nanotechnology, a highly relevant nanomaterial, has been utilized as a new material in advanced sensor detection. In this study, colorimetric methods based on peroxidase enzymes were developed for measuring H 2 O 2 . The synthesized gold nanoparticles (AuNPs) showed a concentration of approximately 1.73 nM at a wavelength of 520 nm. The average diameter displayed a uniform size distribution, estimated at 18 nm, and an increase in the shell thickness of AuNPs-horseradish peroxidase (HRP) was observed in the TEM images. The AuNPs-HRP system demonstrated remarkable catalytic activity in the reaction of the chromogenic substrate tetramethylbenzidine (TMB) with H 2 O 2 , resulting in the production of an oxide product. The optimal conditions for the AuNPs-HRP system, as determined by central composite design (CCD), were a temperature of 25 °C and a pH of 7 within an 8 h period. A strong linear relationship was observed between different absorbance values and the H 2 O 2 concentration, with a coefficient of determination of 0.9956. A portable platform was successfully used to determine H 2 O 2 levels in beverages with recoveries ranging from 95.51% to 118.85%. These findings suggest that the AuNPs-HRP system could be applied to detect H 2 O 2 in beverages.
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