Highly Electroactive Tissue Engineering Scaffolds Based on Nanocellulose/Sulfonated Carbon Nanotube Composite Hydrogels for Myocardial Tissue Repair

Myocardial infarction (MI) has been a serious threat to the health of modern people for a long time. The introduction of tissue engineering (TE) therapy into the treatment of MI is one of the most promising therapeutic schedules. Considering the intrinsic electrophysiological activity of cardiac tissue, we utilized 2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO)-oxidized cellulose nanofibrils (TOCNs) with excellent biocompatibility as the substrate, and sulfonated carbon nanotubes (SCNTs) with remarkable conductivity and water dispersibility as the electrically active material, to prepare TOCN-SCNT composite hydrogels. By adjusting the content of SCNTs from 0 to 5 wt %, TOCN-SCNT hydrogels exhibited conductivity ranging from 5.2 × 10^-6 to 6.2 × 10^-2 S cm^-1. Just with 1 wt % incorporation of SCNTs, the hydrogel played a role in promoting the adhesive growth and proliferation of cells. The hydrogel expressed higher Connexin 43 (Cx-43) and cardiac troponin-T proteins compared with controls, demonstrating great potential in constructing a myocardial TE scaffold.