跨膜蛋白
平移(音频)
计算生物学
抗体
G蛋白偶联受体
膜蛋白
细胞
跨膜结构域
药物发现
生物
抗原
受体
细胞生物学
生物信息学
膜
信号转导
生物化学
免疫学
镜头(地质)
缩放
古生物学
作者
Patrick J. Krohl,Justyn Fine,Yang Hui-lin,Derek VanDyke,Zhiwei Ang,Kook Bum Kim,Andrei Thomas-Tikhonenko,Jamie B. Spangler
标识
DOI:10.1016/j.crmeth.2023.100429
摘要
Due to their critical functions in cell sensing and signal processing, membrane proteins are highly preferred as pharmacological targets, and antibody drugs constitute the fastest growing category of therapeutic agents on the pharmaceutical market. However, major limitations exist in developing antibodies that recognize complex, multipass transmembrane proteins, such as G-protein-coupled receptors (GPCRs). These challenges, largely due to difficulties with recombinant expression of multipass transmembrane proteins, can be overcome using whole-cell screening techniques, which enable presentation of the functional antigen in its native conformation. Here, we developed suspension cell-based whole-cell panning methodologies to screen for specific binders against GPCRs within a naive yeast-displayed antibody library. We implemented our strategy to discover high-affinity antibodies against four distinct GPCR target proteins, demonstrating the potential for our cell-based screening workflow to advance the discovery of antibody therapeutics targeting membrane proteins.
科研通智能强力驱动
Strongly Powered by AbleSci AI