清脆的
分子诊断学
食源性病原体
纳米技术
生化工程
食品安全
生物技术
食品微生物学
计算生物学
生物
细菌
生物信息学
食品科学
材料科学
工程类
基因
生物化学
单核细胞增生李斯特菌
遗传学
作者
Yunhao Lu,Hao Yang,Jinrong Bai,Qiang He,Ruijie Deng
标识
DOI:10.1080/10408398.2022.2153792
摘要
Foodborne pathogenic infection has brought multifaceted issues to human life, leading to an urgent demand for advanced detection technologies. CRISPR/Cas-based biosensors have the potential to address various challenges that exist in conventional assays such as insensitivity, long turnaround time and complex pretreatments. In this perspective, we review the relevant strategies of CRISPR/Cas-assisted diagnostics on foodborne pathogens, focusing on biosensing platforms for foodborne pathogens based on fluorescence, colorimetric, (electro)chemiluminescence, electrochemical, and surface-enhanced Raman scattering detection. It summarizes their detection principles by the clarification of foodborne pathogenic bacteria, fungi, and viruses. Finally, we discuss the current challenges or technical barriers of these methods against broad application, and put forward alternative solutions to improve CRISPR/Cas potential for food safety.
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