“Three-in-one” nanocomposites as multifunctional nanozymes for ultrasensitive ratiometric fluorescence detection of alkaline phosphatase

. Furthermore, this bioassay can detect ALP in mixture and human serum samples, presenting good selectivity as well as real-world applicability. This work not only provides a novel approach for the preparation of a multiple-enzyme-like nanozyme but also offers an advanced ratiometric fluorescence sensing platform for ultrasensitive bioanalysis.