Anti-lung cancer therapy using nano-assembly particles of traditional Chinese Medicine formula

Nano-assembly particles (NAPs) isolated from Traditional Chinese Medicine (TCM) formulations retain a better multi-component overall effect than isolated single active ingredient, which gradually emerging as a transformative approach for the pharmacodynamic and pharmacological research of TCM. This study introduced an innovative research paradigm for investigating the mechanisms of TCM formulas and proposes the groundbreaking concept of "TCM nano-formulated prescriptions" using Yi-Fei-San-Jie Formula (YFSJF) as a clinically study model. Firstly, based on the favorable outcomes in 31 clinical patients with pulmonary space-occupying lesions who received YFSJF treatment, the anti-tumor efficacy of NAPs isolated from the YFSJF decoction was systematically evaluated. Furthermore, the basic material components and underlying mechanism of NAPs were elucidated. Finally, 248 artificially synthetic nanoparticles developed by central bioactive components of YFSJF were used to study the self-assembly process, anti-tumor activity and safety profile. This study firstly demonstrated the potential NAPs-based replacement of traditional formulas and establishes a pioneering methodology for screening multi-component NAPs. • Discovers NAPs in YFSJF decoction as primary anti-tumor components. • Proposes "TCM nano-formulated prescriptions" via novel NAPs research paradigm. • Shows extracted YFSJF NAPs match crude formula efficacy in vivo. • Elucidates NAPs mechanism via integrated transcriptomics & mass spectrometry. • Validates synthetic NAPs replace traditional formula, enabling new screening.