Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis

Article23 March 2023 Source DataTransparent process Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis Jia-Qian Ju Jia-Qian Ju orcid.org/0009-0004-7599-6065 College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China Contribution: Conceptualization, Data curation, Formal analysis, ​Investigation, Methodology, Writing - original draft Search for more papers by this author Zhen-Nan Pan Zhen-Nan Pan orcid.org/0000-0001-9603-0682 College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China Contribution: Resources, Data curation, Software, Formal analysis, Methodology Search for more papers by this author Kun-Huan Zhang Kun-Huan Zhang orcid.org/0009-0008-2170-330X College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China Contribution: Resources, Software, Methodology Search for more papers by this author Yi-Ming Ji Yi-Ming Ji College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China Contribution: Resources, Software, Methodology Search for more papers by this author Jing-Cai Liu Jing-Cai Liu orcid.org/0000-0001-8917-4231 College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China Contribution: Data curation, Software, Formal analysis, Methodology Search for more papers by this author Shao-Chen Sun Corresponding Author Shao-Chen Sun [email protected] orcid.org/0000-0001-5060-1742 College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China Contribution: Conceptualization, Supervision, Funding acquisition, Project administration, Writing - review & editing Search for more papers by this author Jia-Qian Ju Jia-Qian Ju orcid.org/0009-0004-7599-6065 College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China Contribution: Conceptualization, Data curation, Formal analysis, ​Investigation, Methodology, Writing - original draft Search for more papers by this author Zhen-Nan Pan Zhen-Nan Pan orcid.org/0000-0001-9603-0682 College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China Contribution: Resources, Data curation, Software, Formal analysis, Methodology Search for more papers by this author Kun-Huan Zhang Kun-Huan Zhang orcid.org/0009-0008-2170-330X College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China Contribution: Resources, Software, Methodology Search for more papers by this author Yi-Ming Ji Yi-Ming Ji College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China Contribution: Resources, Software, Methodology Search for more papers by this author Jing-Cai Liu Jing-Cai Liu orcid.org/0000-0001-8917-4231 College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China Contribution: Data curation, Software, Formal analysis, Methodology Search for more papers by this author Shao-Chen Sun Corresponding Author Shao-Chen Sun [email protected] orcid.org/0000-0001-5060-1742 College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China Contribution: Conceptualization, Supervision, Funding acquisition, Project administration, Writing - review & editing Search for more papers by this author Author Information Jia-Qian Ju1, Zhen-Nan Pan1, Kun-Huan Zhang1, Yi-Ming Ji1, Jing-Cai Liu1 and Shao-Chen Sun *,1 1College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China *Corresponding author. Tel/Fax: +86 25 84399092; E-mail: [email protected] EMBO Reports (2023)e56273https://doi.org/10.15252/embr.202256273 Full textView the full text of the articlePDFDownload PDF of article text and main figures. Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Abstract Microspherule protein 1 (Mcrs1) is a component of the nonspecific lethal (NSL) complex and the chromatin remodeling INO80 complex, which participates in transcriptional regulation during mitosis. Here, we investigate the roles of Mcrs1 during female meiosis in mice. We demonstrate that Mcrs1 is a novel regulator of the meiotic G2/M transition and spindle assembly in mouse oocytes. Mcrs1 is present in the nucleus and associates with spindle poles and chromosomes of oocytes during meiosis I. Depletion of Mcrs1 alters HDAC2-mediated H4K16ac, H3K4me2, and H3K9me2 levels in nonsurrounded nucleolus (NSN)-type oocytes, and reduces CDK1 activity and cyclin B1 accumulation, leading to G2/M transition delay. Furthermore, Mcrs1 depletion results in abnormal spindle assembly due to reduced Aurora kinase (Aurka and Aurkc) and Kif2A activities, suggesting that Mcrs1 also plays a transcription-independent role in regulation of metaphase I oocytes. Taken together, our results demonstrate that the transcription factor Mcrs1 has important roles in cell cycle regulation and spindle assembly in mouse oocyte meiosis. Synopsis Microspherule protein 1 (Mcrs1) is involved in meiotic resumption in female mice by regulating histone modifications and the maturation promotion factor (MPF) in germinal vesicle (GV)-stage oocytes. It also interacts with Aurora kinases to regulate spindle assembly during metaphase I. Mcrs1 regulates CDK1 activity and cyclin B1 accumulation in oocyte meiosis. Mcrs1 regulates histone modifications and transcriptional activity in GV oocytes. Mcrs1 regulates meiotic spindle assembly via the Aurora kinases in oocytes. Previous ArticleNext Article Read MoreAbout the coverClose modalView large imageVolume 0,Issue 0,This month's cover highlights the article Ubiquitylation of BBSome is required for ciliary assembly and signaling by Francesco Chiuso, Rossella Delle Donne, Ivan Conte, Antonio Feliciello and colleagues. The images show the expression of the BBSome component BBS1 (green), wt (left panels) and an ubiquitylation‐defective BBS1 mutant (right panels), namely BBS1K143R, in differentiating rods (immunostained for Rhodopsin, red). DAPI staining is shown in blue. Expression of the mutant impairs ciliogenesis and photoreceptor morphogenesis, thus recapitulating Bardet Biedl Syndrome (BBS) phenotypes in a medaka fish model.(Scientific image by Giuliana Giamundo and Daniela Intartaglia, University of Naples Federico II.) 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