生物
副热带青蟹
转录组
免疫系统
代谢途径
微生物学
糖酵解
乳酸脱氢酶
肝胰腺
代谢组学
病菌
生物化学
酶
基因
免疫学
基因表达
生物信息学
作者
Chang-Hong Cheng,Hongling Ma,Guangxin Liu,Yiqing Deng,Jianjun Jiang,Feng Jiang,Zhiyong Guo
标识
DOI:10.1016/j.fsi.2022.06.058
摘要
Mud crab reovirus (MCRV) is a serious pathogen that leads to large economic losses in the mud crab farming. However, the molecular mechanism of the immune response after MCRV infection is unclear. In the present study, physiological, transcriptomic, and metabolomic responses after MCRV infection were investigated. The results showed that MCRV infection could increase lactate dehydrogenase, alanine aminotransferase, and aspartate aminotransferase activities. MCRV infection decreased antioxidant enzyme activity levels, induced oxidative stress, and caused severe histological damage. Transcriptome analysis identified 416 differentially expressed genes, including 354 up-regulated and 62 down-regulated genes. The detoxification, immune response, and metabolic processes-related genes were found. The results showed that two key pathways including phagocytosis and apoptosis played important roles in response to MCRV infection. The combination of transcriptomic and metabolomic analyses showed that related metabolic pathways, such as glycolysis, citrate cycle, lipid, and amino acid metabolism were also significantly disrupted. Moreover, the biosynthesis of unsaturated fatty acids was activated in response to MCRV infection. This study provided a novel insight into the understanding of cellular mechanisms in crustaceans against viral invasion.
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