产甲烷
甲烷
甲烷厌氧氧化
环境化学
化学
代谢途径
古细菌
硫黄
微观世界
微生物种群生物学
新陈代谢
生物化学
生物
细菌
有机化学
基因
遗传学
作者
Zifang Chi,Yuhuan Zhu,Ying Yin
标识
DOI:10.1016/j.wasman.2022.01.032
摘要
In anaerobic landfill, SO42- could serve as electron receptor for methane oxidation. In theory, concentrations of both methane and SO42- should be related to methane oxidation rate. However, the dynamics process has yet to be discovered, and the understanding of metabolic pathways of the sulfate-dependent anaerobic methane oxidation (S-DAMO) process in landfill remains limited. In this study, S-DAMO dynamics was investigated by observing the CH4 oxidation rates under different CH4/ SO42-counter-gradients. The CH4-SO42- dual-substrate model based on MichaeliseMenten equation was got (maximum substrate degradation rate Vmax [22.9 ± 1.31] µmol/[kg·d], half-saturation constants [Formula: see text] , and [Formula: see text] ). High-throughput sequencing analysis indicated Methanobacterials, Methanosarcinales, and Soil Crenarchaeotic were the main functional microorganisms for S-DAMO in landfill. The metabolic pathway of S-DAMO was speculated as the reverse methanogenesis pathway through Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUST) analysis, while methanogenesis was the methyl nutrition way based on methanol. The enzymes related to the carbon and sulfur cycles and their relative abundances in the microcosms were analyzed to graph the methane metabolic pathway and the sulfur metabolic pathway. The findings provide important parameters for CH4 mitigation in landfills, and give a new insight for understanding S-DAMO metabolic pathway in landfill.
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