基因组
生物
温泉
嗜热菌
超嗜热菌
生物膜
微生物垫
糖苷水解酶
微生物种群生物学
纤维素酶
微生物
微生物学
古细菌
细菌
植物
酶
生物化学
基因
遗传学
蓝藻
古生物学
作者
Kok Jun Liew,Chee Hung Liang,Yee Ting Lau,Amira Suriaty Yaakop,Kok‐Gan Chan,Saleha Shahar,Mohd Shahir Shamsir,Kian Mau Goh
标识
DOI:10.1038/s41598-022-06943-9
摘要
The SKY hot spring is a unique site filled with a thick layer of plant litter. With the advancement of next-generation sequencing, it is now possible to mine many new biocatalyst sequences. In this study, we aimed to (i) identify the metataxonomic of prokaryotes and eukaryotes in microbial mats using 16S and 18S rRNA markers, (ii) and explore carbohydrate degrading enzymes (CAZymes) that have a high potential for future applications. Green microbial mat, predominantly photosynthetic bacteria, was attached to submerged or floating leaves litter. At the spring head, the sediment mixture consisted of plant debris, predominantly brownish-reddish gelatinous microbial mat, pale tan biofilm, and grey-white filament biofilm. The population in the spring head had a higher percentage of archaea and hyperthermophiles than the green mat. Concurrently, we cataloged nearly 10,000 sequences of CAZymes in both green and brown biofilms using the shotgun metagenomic sequencing approach. These sequences include β-glucosidase, cellulase, xylanase, α-N-arabinofuranosidase, α-L-arabinofuranosidase, and other CAZymes. In conclusion, this work elucidated that SKY is a unique hot spring due to its rich lignocellulosic material, often absent in other hot springs. The data collected from this study serves as a repository of new thermostable macromolecules, in particular families of glycoside hydrolases.
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