免疫金标记
电子显微镜
超微结构
抗原性
免疫电镜
低温电子显微
过氧化物酶体
显微镜
细胞生物学
冷冻固定
化学
生物物理学
生物
生物化学
病理
解剖
物理
光学
抗体
医学
遗传学
基因
作者
Karina Mildner,Dagmar Zeuschner
出处
期刊:Methods in molecular biology
日期:2017-01-01
卷期号:: 101-111
标识
DOI:10.1007/978-1-4939-6937-1_11
摘要
Electron microscopy samples processed for cryo-immunogold-labeling need to be gently fixed to keep their antigenicity. Biological material like cultured cells or tissue can be prepared according to the standard Tokuyasu fixation or in a further developed rehydration method based on high-pressure freezing. We will describe here the variant and common steps of both methods in detail and illustrate their potency in the ultrastructural imaging of peroxisomes.
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