醛脱氢酶
干细胞
造血
川地34
癌症研究
CD38
生物
白血病
CD19
分子生物学
免疫学
免疫系统
酶
生物化学
细胞生物学
作者
Wenwen Wang,Elena Foerner,Eike C. Buss,Anna Jauch,Volker Eckstein,Patrick Wuchter,Anthony D. Ho,Christoph Lutz
标识
DOI:10.1080/10428194.2016.1236378
摘要
In B-cell acute lymphoblastic leukemia (B-ALL) separation of normal hematopoietic stem cells (HSC) has so far been limited to a subgroup of patients. As aldehyde dehydrogenase (ALDH)-activity is enriched in various stem cells we investigated its value for HSC isolation in adult B-ALL. Based on ALDH-activity patients could be stratified in ALDH-numerous (≥1.9% ALDH+ cells) and ALDH-rare (<1.9% ALDH+ cells) cases. In ALDH-rare B-ALL clonal-marker negative HSC could be separated by the CD34+CD38−ALDH+ phenotype, whereas this separation was not possible in ALDH-numerous B-ALL. Functional analysis confirmed the HSC-potential of isolated cells, which were uniformly CD19-negative. However, addition of ALDH-activity further improved HSC-purity. In summary, we provide a method to separate functionally normal HSC from leukemic cells in a subgroup of B-ALL patients that can be identified prospectively. This protocol thereby facilitates comparative analyses of matched HSC and leukemic cells in order to improve our understanding of leukemia evolution.
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