获得性免疫系统
免疫系统
先天免疫系统
CD8型
免疫
免疫学
佐剂
淋巴
淋巴结
抗原
生物
医学
病理
作者
Sun‐Young Kim,Young-Woock Noh,Tae Heung Kang,Jung-Eun Kim,Young Ho Kim,Soong Ho Um,Doo-Byoung Oh,Yeong‐Min Park,Yong Taik Lim
出处
期刊:Biomaterials
[Elsevier]
日期:2017-06-01
卷期号:130: 56-66
被引量:115
标识
DOI:10.1016/j.biomaterials.2017.03.034
摘要
In this study, synthetic vaccine nanoparticles (SVNPs) that efficiently targeted lymph nodes, where immune responses against foreign antigens are primed, were developed to enhance antitumor immunity. The size (20-70 nm) and surface character (amination) of poly(γ-glutamic acid)-based SVNPs were selected for effective loading and delivery (i.e., migration and retention) of model tumor antigen (OVA) and toll-like receptor 3 agonist (poly (I:C)) to immune cells in lymph nodes. Antigen-presenting cells treated with SVNP-OVA and SVNP-IC showed higher uptake of OVA and poly (I:C) and higher secretion of inflammatory cytokines (TNF-α, IL-6) and type I interferon (IFN-α, IFN-β) than those treated with OVA and poly (I:C) alone. In vivo analysis revealed higher levels of activation markers, inflammatory cytokines, and type I IFNs in the lymph nodes of mice immunized with SVNP-IC compared to those of mice in other groups. SVNP-IC-treated mice showed significantly greater in vivo natural killer cell expansion/activation (NK1.1+ cells) and CD8+ T cell response (CD8+ INF-γ+ cells) in innate and adaptive immunity, respectively. Both preventive and therapeutic vaccination of EG7-OVA tumor-bearing mice using the simultaneous injection of both SVNP-OVA and SVNP-IC induced higher antitumor immunity and inhibited tumor growth.
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