化学
毛细管电泳
安培法
色谱法
褪黑素
栏(排版)
毛细管作用
分析化学(期刊)
电极
电化学
气象学
内科学
医学
物理化学
物理
结构工程
连接(主束)
工程类
作者
Tianyan You,Zhiming Liu,Xiurong Yang,Erkang Wang
出处
期刊:Talanta
[Elsevier]
日期:1999-07-01
卷期号:49 (3): 517-521
被引量:17
标识
DOI:10.1016/s0039-9140(99)00042-9
摘要
A rapid and sensitive method is described for the detection of melatonin in rat pineal gland and melatonin tablets. Capillary electrophoresis (CE) with electrochemical detection (EC) was used. This method had high sensitivity and good reproducibility. The detection limit of melatonin was as low as 1.3×10−9 mol/l (0.30 fg). There was a linear range of concentration from 3.9×10−7 to 3.25×10−5 mol/l with a correlation coefficient of 0.999. The linear equation was Y=8.27+0.0042X with a slope of 0.0042 pA/nM. The relative standard deviations of the peak current response and the migration time for 16 continuous injections of 6.5 μM melatonin were 4.5 and 0.48%, respectively. The proposed method was applied to detect melatonin in the rat pineal gland and the melatonin tablets. Good results were obtained compared with previous reports.
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