微流控
三维细胞培养
微量滴定板
细胞培养
细胞
芯片上器官
细胞生物学
效价
计算生物学
纳米技术
生物
化学
材料科学
分子生物学
免疫学
生物化学
遗传学
抗体
作者
Sebastiaan J. Trietsch,Guido D. Israëls,Jos Joore,Thomas Hankemeier,Paul Vulto
出处
期刊:Lab on a Chip
[The Royal Society of Chemistry]
日期:2013-01-01
卷期号:13 (18): 3548-3548
被引量:156
摘要
Human tissues and organs are inherently heterogeneous. Their functionality is determined by the interplay between different cell types, their secondary architecture, vascular system and gradients of signaling molecules and metabolites. Here we propose a stratified 3D cell culture platform, in which adjacent lanes of gels and liquids are patterned by phaseguides to capture this tissue heterogeneity. We demonstrate 3D cell culture of HepG2 hepatocytes under continuous perfusion, a rifampicin toxicity assay and co-culture with fibroblasts. 4T1 breast cancer cells are used to demonstrate invasion and aggregation models. The platform is incorporated in a microtiter plate format that renders it fully compatible with automation and high-content screening equipment. The extended functionality, ease of handling and full compatibility to standard equipment is an important step towards adoption of Organ-on-a-Chip technology for screening in an industrial setting.
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