Microbial diversity investigation of dry fermentation of rice stalk was applied 454-pyrosequencing technology. The results suggested that clone library deriving a well bacteria community's coverage. Eight phylogenetic groups at the taxonomic rank of phylum were derived which including Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria, Chloroflexi, Spirochaetes, Synergistetes and Unclassified Bacteria. Firmicutes (1953 sequences) was the most dominant microbial communities which accounted for up to 92.5% of all total bacteria communities. Genus Sporacetigenium was the predominant bacteria community which accounted for up to 58.6%, genus Turicibacter (13.8%) and Clostridiaceae (15.9%) were the second predominant bacteria communities.