胚乳
赤霉素
生物
突变体
胚胎
水稻
颖果
发芽
基因表达
分子生物学
基因
细胞生物学
生物化学
植物
作者
Miyuki Kaneko,Hironori Itoh,Miyako Ueguchi‐Tanaka,Motoyuki Ashikari,Makoto Matsuoka
出处
期刊:Plant Physiology
[Oxford University Press]
日期:2002-04-01
卷期号:128 (4): 1264-1270
被引量:198
摘要
Abstract We recently isolated two genes (OsGA3ox1 andOsGA3ox2) from rice (Oryza sativa) encoding 3β-hydroxylase, which catalyzes the final step of active gibberellin (GA) biosynthesis (H. Itoh, M. Ueguchi-Tanaka, N. Sentoku, H. Kitano, M. Matsuoka, M. Kobayashi [2001] Proc Natl Acad Sci USA 98: 8909–8914). Using these cloned cDNAs, we analyzed the temporal and spatial expression patterns of the 3β-hydroxylase genes and also an α-amylase gene (RAmy1A) during rice seed germination to investigate the relationship between GA biosynthesis and α-amylase expression. Northern-blot analyses revealed that RAmy1Aexpression in the embryo occurs before the induction of 3β-hydroxylase expression, whereas in the endosperm, a high level ofRAmy1A expression occurs 1 to 2 d after the peak ofOsGA3ox2 expression and only in the absence of uniconazol. Based on the analysis of an OsGA3ox2 null mutant (d18-Akibare dwarf), we determined that 3β-hydroxylase produced by OsGA3ox2 is important for the induction of RAmy1A expression and that the OsGA3ox1 product is not essential for α-amylase induction. The expression ofOsGA3ox2 was localized to the shoot region and epithelium of the embryo, strongly suggesting that active GA biosynthesis occurs in these two regions. The synthesis of active GA in the epithelium is important for α-amylase expression in the endosperm, because an embryonic mutant defective in shoot formation, but which developed epithelium cells, induced α-amylase expression in the endosperm, whereas a mutant defective in epithelium development did not.
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