Phase retrieval method for biological samples with absorption

Interferometric microscopy is an important technique to obtain the phase distribution of cells and tissues. However, in practice, the absorption effect of the biological samples and non-uniformities in the beam could induce errors in solving phase distributions by traditional methods. In this paper, an improved phase retrieval method is introduced to quantitatively calculate the phase distribution of absorbing samples. The proposed method could acquire more accurate phase distributions and optical transmitted sample images physically. Comparing with conventional phase retrieval methods, the proposed method has higher accuracy and calculation efficiency.