材料科学
生理盐水
微球
体内分布
生物医学工程
泊洛沙姆
基质凝胶
自愈水凝胶
泄漏(经济)
医学
麻醉
化学
内科学
复合材料
体外
生物化学
化学工程
共聚物
血管生成
聚合物
高分子化学
经济
宏观经济学
工程类
作者
Jeff N. Anderl,Thomas E. Robey,Patrick S. Stayton,Charles E. Murry
摘要
Abstract Intramyocardial injection of therapeutic agents may enhance heart repair after infarction. Incomplete retention of intramyocardial injections has been reported, but modes of loss are undefined. We determined the fate of neutron‐activated microspheres injected into acutely ischemic rat myocardium using saline, Pluronic F127, or Matrigel as vehicle. Twenty minutes after injection in saline, 63% ± 12% of 10‐μm microspheres was retained in the heart. Similar retention was observed after 6 days. Injection site leakage accounted for 14% ± 5% of the microspheres, whereas exit via coronary veins resulted in 11.2% ± 9.5% collecting in the lungs. Microspheres distribution to other organs was minimal. Retention of 40‐μm microspheres was similar to that observed with the 10‐μm microspheres. Pluronic F127 and Matrigel reduced immediate leakage to 4% ± 1% and 2% ± 1%, respectively. Surprisingly, microsphere retention in the heart was not improved at 20 min using either gelling vehicle, suggesting that leakage occurs over a prolonged period. Thus, most injected particles are retained in the ischemic rat heart following direct injection, but significant fractions are lost from the injection site and through coronary veins. Gelling agents reduced short‐term leakage, but failed to enhance longer‐term retention. Hydrogels with stiffer mechanical properties might enhance retention and reduce variability. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009
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