链霉亲和素
分子生物学
核酸外切酶 III
化学
生物
分离(微生物学)
DNA提取
DNA
生物素
聚合酶链反应
生物化学
基因
大肠杆菌
生物信息学
作者
Yuji Wakimoto,Jianming Jiang,Hiroko Wakimoto
标识
DOI:10.1002/0471142727.mb0215s107
摘要
Single-stranded DNA (ssDNA) is often used for DNA sequencing as well as microarray and hybridization technologies. Asymmetric PCR or exonuclease digestion followed by urea gel separation and isolation on streptavidin-coated magnetic beads are commonly used for this purpose. These two methods may not yield large amounts of highly purified ssDNA. This protocol for ssDNA isolation from PCR-amplified DNA involves biotin labeling of one strand and subsequent strand separation utilizing streptavidin-coated magnetic beads.
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