生物传感器
检出限
DNA
材料科学
HpaII公司
辣根过氧化物酶
DNA甲基化
限制性酶
线性范围
电极
分子生物学
纳米技术
生物
基因
化学
生物化学
色谱法
酶
基因表达
物理化学
作者
Xi Chen,Jian Huang,Shu Zhang,Fei Mo,Shasha Su,Yan Li,Lichao Fang,Jun Deng,Hui Huang,Zhen Luo,Junsong Zheng
标识
DOI:10.1021/acsami.8b20144
摘要
DNA methylation is a key factor in the pathogenesis of gene expression diseases or malignancies. Thus, it has become a significant biomarker for the diagnosis and prognosis of these diseases. In this paper, we designed an ultrasensitive and specific electrochemical biosensor for DNA methylation detection. The platform consisted of stem-loop-tetrahedron composite DNA probes anchoring at a Au nanoparticle-coated gold electrode, a restriction enzyme digestion of HpaII, and signal amplification procedures including electrodeposition of Au nanoparticles, hybridization chain reaction, and horseradish peroxidase enzymatic catalysis. Under optimal conditions, the design showed a broad dynamic range from 1 aM to 1 pM and a detection limit of about 0.93 aM. The approach also showed ideal specificity, repeatability, and stability. The recovery test demonstrated that the design is a promising platform for DNA methylation detection under clinical circumstances and could meet the need for cancer diagnosis.
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