Assessment of Novel C-4 Methylated Tetraketide by HPLC-SPE-TT in Saccharicola sp., an Endophytic Fungus in Eugenia jambolana Lam.

A new compound named methyl (6S,7S,2E,4E)-6,7-dihydroxy-4,6-dimethyl octanoate was isolated from Saccharicola sp. using high performance liquid chromatography-solid phase extraction-transfer tube (HPLC-SPE-TT), together with four known compounds, fusaric acid, trans-4-hydroxymellein, thymidine and adenosine.Saccharicola sp. was isolated from the stems of Eugenia jambolana Lam.(Myrtaceae) as endophytic fungus.The structure of this novel substance was established based on its spectroscopic data, including 1 H and 13 C nuclear magnetic resonance (NMR), 2D NMR techniques and high-resolution electrospray ionization mass spectrometry (HR-ESI-MS).This paper deals with the first report of its kind on this compound in Saccharicola sp.