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酿酒酵母
基因组
生物
蛋白质组
蛋白质标签
计算生物学
遗传学
异源的
基因组文库
高通量筛选
酵母
基因
肽序列
融合蛋白
重组DNA
作者
Matthias Meurer,Yuanqiang Duan,Ehud Sass,Ilia Kats,Konrad Herbst,Benjamin C. Buchmuller,Verena Dederer,Florian Huber,Daniel Kirrmaier,Martin Štefl,Koen Van Laer,Tobias P. Dick,Marius K. Lemberg,Anton Khmelinskii,Emmanuel D. Levy,Michael Knop
出处
期刊:Nature Methods
[Springer Nature]
日期:2018-07-09
卷期号:15 (8): 598-600
被引量:41
标识
DOI:10.1038/s41592-018-0045-8
摘要
Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces cerevisiae open reading frames (ORFs). In 5,661 strains, we inserted an acceptor module after each ORF that can be efficiently replaced with tags or regulatory elements. We validated the library with targeted sequencing and tagged the proteome with bright fluorescent proteins to quantify the effect of heterologous transcription terminators on protein expression and to localize previously undetected proteins.
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