凝血酶
化学
电极
光电流
纳米技术
检出限
印版阅读器
pH计
吞吐量
生物传感器
光电子学
色谱法
材料科学
光学
无线
计算机科学
生物化学
电信
物理
物理化学
免疫学
荧光
生物
血小板
作者
Juan Wang,Mengmeng Song,Chengguo Hu,Kangbing Wu
标识
DOI:10.1021/acs.analchem.8b01979
摘要
In this work, a self-powered, portable, and light-addressable photoelectrochemical sensor (P-LAPECS) is developed for efficient drug screening using a handheld pH meter readout. The sensor, which employs thrombin inhibitors as the drug model, is constructed by evenly immobilizing biotin-labeled and thrombin-cleavable peptides on eight separated sensing zones of a single gold film electrode. The incubation of each peptide sensing zone with thrombin leads to the reduction of binding sites for streptavidin-labeled fullerene (C60) PEC bioprobes, which directly reflects the activity of thrombin by the variation of both photocurrent and photovoltage, and therefore allows the screening of thrombin inhibitors using either a single-channel electrochemical analyzer or a portable pH meter. Consequenty, the inhibition efficiency evaluation of multiple thrombin inhibitors can be achieved by just one electrode, and the screening result obtained by the pH meter is very close to that acquired by the electrochemical analyzer. Moreover, P-LAPECS can realize the light-addressable detection of thrombin with a detection limit as low as 0.05 pM. The present work thus demonstrates the possibility of constructing portable, inexpensive, sensitive, and high-throughput biosensing platforms using ubiquitous pH meters for laboratories all over the world.
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