Effects of cryoprotectants and vitrification on in vitro development ability of porcine oocytes at MII stage

Vitrification freezing methods were used to filtrate seven vitrification solutions and compare effects of three carriers on freezing oocytes by using MII oocytes in the present project. The results showed seven groups of cryoprotectants had low toxicity on MII oocytes. The rate of split and blastocyst,the number of blastocyst cells in each group was lower than those in the control group. Three groups of cryoprotectant with smallest damage to MII oocytes development ability was selected to compare vitrification by GMP. The results indicated that EFS was not suitable for MII oocytes vitrification,which the 1. 19% normal rate and 0 cleavage rate was significant different( P 0. 05) from other two groups( 59. 48%,53. 55%; 24. 19%,35. 02%). HM + 7. 5%( DMSO + EG),HM + 17%( DMSO + EG) + 0. 4 mol / L Su was selected as the cryoprotectant to compare the effect of three carriers. The data showed that hemi-straw( 53. 67%) was significant different( P 0. 05) from OPS( 29. 33%) and GMP( 35. 00%) in cleavage rate. Together,the hemi-straw was more suitable for MII porcine oocytes vitrification.