Ratio of alliin/alliinase in catalytic system of garlic.

To obtain effective conversion of alliin to allicin in vivo,a suitable ratio of alliin/alliinase delivery system was investigated in vitro.An HPLC method was developed to determine the conversion rates of alliin and the production rates of allicin in the catalytic system.When the weight ratio of alliin/alliinase was 1∶1 with the alliinase of 1 000 U/g,the conversion rate of alliin was(95.7±1.2)% and the production rate of allicin was(87.4±1.3)%.Data from this study on the ratio of alliin/alliinase could be applied in the development of alliin/alliinase preparation.In the alliin/alliinase catalytic system,1 mg alliin could be catalyzed by 1.3 U alliinase to produce allicin efficiently.