Establishment and Application of a Multiplex PCR for Detecting Porcine Parvovirus,Pseudorabies Virus and Porcine circovirus type2

A multiplex polymerase chain reaction(PCR)-based assay was developed and evaluated for its ability to simultaneously detect multiple viral infections of swine.Specific primers for detecting porcine parvovirus(PPV),pseudorabies virus(PRV) and porcine circovirus type 2(PCV-2) were designed according to the full sequences of each virus strain obtained from GenBank.A multiplex PCR was established to detect PPV,PRV and PCV-2 by optimizing the PCR programs.286 blood and tissues samples collected in clinical healthy pig population and disease pigs of pig farms in Shaanxi were detected using developed multiplex PCR for screening PPV,PRV and PCV-2.The results indicated that the PCV-2 was an important pathogen in clinical healthy pig population,the PRV and PPV also could be found in individual pig farms,so it was important issue for getting rid of the infected pigs from normal pig population.It was serous of PRV+PCV-2 combined infection in diseased pigs,PPV+PRV+PCV-2 combined infection also were detected in diseased pigs.The detected results of multiplex PCR were more than 99% consistent with special single PCR.This developed novel multiplex PCR will be a tool for detecting PPV,PRV and PCV-2 in clinical samples and disease diagnosis.