Determination the Contents of Salidroside and Tyrosol in Rhodiola crenulata from Qinghai

Objective:To establish an HPLC method for determinating the contents of salidroside and tyrosol in Rhodiola crenulata from Qinghai province,China.Method:All the 25 batches of samples were collected from 2007 to 2009.The HPLC analysis was performed on a C18 column,and methanol-water(9.5:90.5) was as mobile phase and the detection wavelengths were 275 nm.Result:The contents of salidroside and tyrosol were 0.102%1.16% and 0.026% 0.095%,respectively.Conclusion:The contents of salidroside and tyrosol varied from sample to sample.