Effects of Culture Conditions on Cell Growth and Total Alkaloid Formation in Suspension Cultures of Pinellia ternata

A study on the changes of cell yield and the effect of carbohydrates,calcium,Fe and inositol on the cell growth and alkaloids synthesis in suspension cultures of Pinellia ternata(Thunb.)Breit.was carried out.The result showed that cell growth process in suspension culture presented a S-shaped curve,the date for harvest yield was about 21~(st) day.Glucose was better than sucrose for cell growth rate and alkaloids synthesis,the optimum concentration was 20?g/L.The optimum MS medium for cell growth rate and alkaloids production was:20?g/L glucose+100?mg/L inositol+1.8?mmol/L CaCl 2·2H 2O+0.06?mmol/L FeSO 4·7H 2O.