IN VITRO CULTURE OF PLASMODIUM FALCIPARUM STRAIN RESISTING ARTESUNATE IN YUNNAN PROVINCE

To culture Plasmodium falciparum strain resisting artesunate. The isolated strain collected from patient was cultured in vitro by Trager method. After isolated strain grows normally, different concentrations of artesunate were added into the culturing plates to culture. ID 50 of artesunate was assayed by Rieckmann's in vitro micro technique at different time. Before and after adding drugs for 68 d, 129 d and stopping drug for 67 d, ID 50 of artesunate was 9.6, 30.6, 85.1 and 52.9 nmol/L respectively. The results suggested that P. falciparum strain high resisting artesunate can be cultured by manual work.